Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia.

doi: 10.1002/advs.202206432

Figure Lengend Snippet: Figure 7. DCD is beneficial to AIS recovery and DCD-containing BM-MSC-derived migrasome effectively promotes phagocytosis of macrophages. A– G) Peripheral blood of AIS patients (acute phase, 0–3d after disease onset, n = 16) and healthy controls (HC, n = 8) were collected. (A) Plasma DCD concentration was assessed with ELISA. *p < 0.05, compared with HC by Student’s t-test (mean ± standard deviation). (B) Correlation of clinic parameters and plasma DCD concentration was assessed with Spearman correlation analysis and Point-biserial correlations. *p < 0.05. DM, diabetes mellitus, CHD, coronary heart disease. (C) Representative images of the magnetic resonance diffusion weighted imaging (MR-DWI) of AIS patients with low plasma DCD concentration (DCD ≤3.33 ng ml−1) or high plasma DCD concentration (DCD > 3.33 ng ml−1). (D) Association between plasma DCD concentration with infarct scale was estimated with Spearman correlation analysis. (E) Association between plasma DCD concentration with delta NIHSS (NIHSS at 7d minus NIHSS at 1d) was estimated with Spearman correlation analysis. (F) Representative images of the chest Computed Tomography (CT) of AIS patients with low plasma DCD concentration (DCD ≤3.39 ng ml−1, median of the cohort) or high plasma DCD concentration (DCD > 3.39 ng ml−1, median of the cohort). (G) Pie charts showing the occurrence of post-stroke pneumonia in AIS patients with low and high plasma DCD concentrations. H) DCD (1 ng ml−1), PBS-migrasomes (PBS-M, 50 μg ml−1) or E. Coli-migrasomes (E. Coli-M, 50 μg ml−1) labeled with Dil (red) were treated to BMDM (15 min). Immunostaining of WGA (green) and DCD (withe) in migrasome-treated BMDM was performed. Experiments were repeated for three times. I,J) BMDM were first pre-stimulated with DCD (1 ng ml−1), PBS-M (50 μg ml−1), or E. Coli-M (50 μg ml−1) for overnight then treated with E. Coli (E. Coli : BMDM = 20:1, 1 h). Phagocytic efficiency of BMDM to GFP expressing E. Coli was assessed with flow cytometry (I) and immunostaining (J). Experiments were repeated three times. **p < 0.01, compared with PBS-treated group by one-way ANOVA (mean ± standard deviation).

Article Snippet: Human Monocyte Enrichment and Macrophage Differentiation: Mononucleus cells were isolated from peripheral blood of healthy adults (age = 18–40y) with human peripheral blood monocyte isolation Solution kit (Solarbio, P8680).

Techniques: Derivative Assay, Clinical Proteomics, Concentration Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation, Imaging, Computed Tomography, Labeling, Immunostaining, Expressing, Cytometry

Journal: Cell reports

Article Title: AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages

doi: 10.1016/j.celrep.2023.113067

Figure Lengend Snippet:

Article Snippet: Human: peripheral blood CD14 + monocyte cells , Stemcell Technologies , Cat#70035.

Techniques: Recombinant, Reverse Transcription, Cell Viability Assay, In Situ, SYBR Green Assay, RNA Sequencing, Quantitative RT-PCR, Luciferase, Software

Journal: mAbs

Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates

doi: 10.1080/19420862.2020.1803645

Figure Lengend Snippet: Antibody-mediated Aβ uptake in human cell systems. (a-c) Mean number of Fc Receptors determined using the QuantiBRITE PE kit on two different human macrophage preparations derived from human peripheral CD14+ monocytes (A, n = 1–2) and on the human THP-1 cell line (B, n = 2). Mean ± S.E.M. are provided. (c) Kifunensine high mannose sample (HM-Afuc) has similar dose-dependent Aβ phagocytosis activity as the standard mediated by primary human macrophages (two-way ANOVA for standard vs sample, p >.05; n = 3). Results are presented as the relative ADCP fluorescence unit (RFU) as measured using the trypan blue exclusion method versus antibody concentration. Mean ± S.E.M. and non-linear regression curve fit analysis are shown. (d) HM-Afuc has similar dose-dependent Aβ phagocytosis activity as the standard mediated by the THP-1 cell line (two-way ANOVA for standard vs sample, p >.05; n = 4). Samples were run in duplicate and Aβ-GFP phagocytosis was quantitated by flow cytometry. The mean ± S.E.M. of the percent positive GFP cells of 1000 cells sampled per sample and non-linear regression curve-fit analysis are shown.

Article Snippet: Human peripheral blood CD14+ monocytes (70035, Stem Cell Technologies) were differentiated to macrophages with recombinant M-CSF (216-MC, R&D Systems) using the following media (Advanced RPMI1640 medium, 5% FBS, Penicillin-Streptomycin 1x, L-Glutamine 2 mM, M-CSF 100 ng/ml).

Techniques: Derivative Assay, Activity Assay, Fluorescence, Concentration Assay, Flow Cytometry

Journal: Cell genomics

Article Title: Functional inference of gene regulation using single-cell multi-omics

doi: 10.1016/j.xgen.2022.100166

Figure Lengend Snippet: (A) Schematic highlighting design of stimulation experiment. Human peripheral blood mononuclear cells (PBMCs) were stimulated with DMSO control, lipopolysaccharide (LPS), interferon gamma (IFN-Ɣ), or phorbol myristate acetate (PMA) plus ionomycin for 1 or 6 h with or without a Golgi inhibitor (GI) for the 6-h treatment condition. Cells were then split and profiled using scATAC-seq and scRNA-seq for each condition and time point considered. (B) Total number of cells profiled per condition passing quality control filtering for scATAC and scRNA-seq. (C) Uniform manifold approximation and projection (UMAP) of scATAC-seq cells based on latent semantic indexing (LSI) dimensionality reduction, with cells colored by treatment condition. (D) UMAP of scRNA-seq cells based on principal-component analysis (PCA) dimensionality reduction, with cells colored by treatment condition. (E) UMAPs of scATAC-seq cells (top) and scRNA-seq cells (bottom), highlighting individual conditions under control (6 h) and PMA (1 and 6 h) conditions. (F) Aggregate accessibility profiles for scATAC-seq monocyte cells around genes IFITM3 and HES4 . (G) Distribution of single-cell expression levels based on the imputed scRNA-seq counts for stimulation-specific gene markers shown in (F) per condition for scRNA-seq monocyte cells.

Article Snippet: Human peripheral blood mononuclear cells (PBMCs), Donor1, 24 yo, Female, 100M cells , Allcells , Cat# LP, CR, MNC, 100M; Lot#3003978.

Techniques: Expressing

Journal: Cell genomics

Article Title: Functional inference of gene regulation using single-cell multi-omics

doi: 10.1016/j.xgen.2022.100166

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human peripheral blood mononuclear cells (PBMCs), Donor1, 24 yo, Female, 100M cells , Allcells , Cat# LP, CR, MNC, 100M; Lot#3003978.

Techniques: Recombinant, Software, Generated